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Module 19610 lessonsRxPrep 2023 opportunistic-infection material, reconciled with the NIH Adult and Adolescent OI guideline updated September 9, 2025 and reviewed June 23, 2026

Toxoplasma gondii Encephalitis

Connect latent infection and cellular immune failure to focal CNS disease, then integrate imaging, empiric diagnosis, folate-pathway pharmacology, ART, toxicity, and relapse prevention.

01

Explain how latent Toxoplasma cysts reactivate during advanced HIV immunosuppression.

02

Recognize focal and nonfocal presentations and interpret the major neuroimaging patterns.

03

Use IgG, CSF PCR, empiric response, and tissue diagnosis according to their limits.

04

Apply current primary prophylaxis criteria and select a regimen that also protects against PCP.

05

Calculate weight-based pyrimethamine, sulfadiazine, and TMP-SMX acute regimens.

06

Use leucovorin rescue and monitor marrow, renal, hepatic, dermatologic, and metabolic toxicity.

07

Select alternatives without losing Toxoplasma activity, PCP coverage, or drug absorption.

08

Identify objective response and escalate nonresponse to diagnostic tissue when appropriate.

09

Coordinate ART, edema treatment, seizure care, IRIS assessment, and interaction management.

10

Continue maintenance until durable immune recovery and restart it at the correct threshold.

196.01

Connect Latent Infection to Cellular Immune Failure

TE in advanced HIV is usually reactivation of a latent tissue cyst. A new exposure can cause disease, but host control determines whether infection reaches the brain.

What to learn
  • Tissue cyst
  • CD4 below 100
  • IgG
  • Environmental prevention
CNS evidence pathwayLatent tissue cyst to Focal inflammation
01Latent tissue cystDefine the starting evidence

Do not lose diagnostic context.

02CD4 declineCarry the evidence forward

Do not lose diagnostic context.

03Brain reactivationCarry the evidence forward

Do not lose diagnostic context.

04Focal inflammationOwn the next decision

Document the clinical action.

Place the parasite

Toxoplasma gondii is an intracellular protozoan. Primary infection is often silent and leaves latent cysts in neural and muscle tissue. Severe loss of T-cell control permits replication and necrotizing focal encephalitis.

Define the risk

Risk increases below CD4 100 cells/mm3 and is greatest below 50. Most cases in people with HIV reflect reactivation, so IgG establishes the latent-infection context.

Reduce new acquisition

Avoid raw or undercooked meat and shellfish, wash produce and hands, use gloves with soil, and change cat litter daily with hand protection. Cats can remain in the home and do not need routine testing.

Restore immunity

Effective ART is the durable prevention strategy. Prophylaxis protects the high-risk interval while viral suppression rebuilds cellular control.

0 of 1 answered
01What most commonly produces TE in advanced HIV?
Answer every question to submit.
196.02

Recognize Focal Brain Disease

TE often presents subacutely with headache and focal neurologic dysfunction. Fever can be absent, and imaging is necessary to define lesions, edema, and mass effect.

What to learn
  • Headache
  • Focal deficit
  • Seizure
  • Ring enhancement
  • Basal ganglia
CNS evidence pathwaySubacute headache to Lesion and edema map
01Subacute headacheDefine the starting evidence

Do not lose diagnostic context.

02Focal deficit or seizureCarry the evidence forward

Do not lose diagnostic context.

03Contrast MRICarry the evidence forward

Do not lose diagnostic context.

04Lesion and edema mapOwn the next decision

Document the clinical action.

Read the examination

Headache, hemiparesis, aphasia, visual change, altered cognition, fever, and seizure can appear over days to weeks. Diffuse encephalitis without a focal lesion is less common but can progress rapidly.

Map the lesions

Contrast CT or MRI typically shows multiple ring-enhancing lesions with surrounding edema, often involving the basal ganglia. A single lesion or atypical distribution remains possible.

Measure mass effect

Document edema, ventricular compression, midline shift, herniation risk, and lumbar-puncture safety before invasive testing.

Keep alternatives visible

Primary CNS lymphoma, tuberculosis, fungal infection, pyogenic abscess, metastasis, Chagas disease, and PML can resemble TE. PML more often affects white matter without enhancement or mass effect outside IRIS.

0 of 1 answered
01Which imaging pattern most strongly supports TE in advanced HIV?
Answer every question to submit.
196.03

Build a Response-Aware Diagnosis

Definitive diagnosis needs organism detection, but a measured response to empiric therapy often establishes the working diagnosis when the syndrome, imaging, and IgG agree.

What to learn
  • IgG
  • CSF PCR
  • Empiric response
  • Brain biopsy
  • CNS lymphoma
CNS evidence pathwayIgG and immune context to Response or tissue diagnosis
01IgG and immune contextDefine the starting evidence

Do not lose diagnostic context.

02Imaging patternCarry the evidence forward

Do not lose diagnostic context.

03CSF when safeCarry the evidence forward

Do not lose diagnostic context.

04Response or tissue diagnosisOwn the next decision

Document the clinical action.

Interpret serology

Most people with TE are IgG positive. A negative IgG makes TE unlikely but does not absolutely exclude it. IgM is usually absent in reactivation, and quantitative titers do not measure CNS disease.

Use CSF selectively

When lumbar puncture is safe, CSF PCR is highly specific but only about 50 percent sensitive and becomes less sensitive after therapy. A negative result cannot rule out TE.

Measure the empiric trial

A compatible syndrome, imaging pattern, IgG, and objective improvement on active therapy support presumptive TE. Record a baseline neurologic examination and reassess within 10 to 14 days.

Escalate nonresponse

Strongly consider brain biopsy with atypical evidence, deterioration during the first week, or no improvement by 10 to 14 days. EBV DNA in CSF raises concern for lymphoma but does not diagnose it alone.

Respect steroid confounding

CNS lymphoma can improve temporarily with corticosteroids. Restrict steroids to a genuine mass-effect indication and interpret subsequent response cautiously.

0 of 1 answered
01A patient has typical lesions and positive IgG but no improvement after 14 days of adequate therapy. What is the best next step?
Answer every question to submit.
196.04

Protect the High-Risk Interval

Primary TE prophylaxis requires both latent infection and severe immunosuppression. The preferred regimen also covers PCP.

What to learn
  • IgG positive
  • CD4 below 100
  • TMP-SMX
  • G6PD
  • Immune recovery
CNS evidence pathwayIgG positive to Stop after recovery
01IgG positiveDefine the starting evidence

Do not lose diagnostic context.

02CD4 below 100Carry the evidence forward

Do not lose diagnostic context.

03TMP-SMX DS dailyCarry the evidence forward

Do not lose diagnostic context.

04Stop after recoveryOwn the next decision

Document the clinical action.

Apply the gate

Start prophylaxis when Toxoplasma IgG is positive and CD4 is below 100 cells/mm3. IgG-negative people need exposure counseling and PCP prevention as indicated, not automatic TE prophylaxis.

Prefer daily TMP-SMX

One double-strength tablet daily is preferred and also prevents PCP. DS three times weekly or SS daily are alternatives when full daily dosing is not tolerated.

Build a complete alternative

Dapsone plus pyrimethamine plus leucovorin or atovaquone with or without pyrimethamine and leucovorin can protect against TE and PCP. Test G6PD before dapsone and give atovaquone with food.

Reject false coverage

Aerosolized pentamidine and dapsone alone do not prevent TE. If used for PCP, another agent must address Toxoplasma risk.

Stop and restart correctly

Stop after CD4 exceeds 200 for more than 3 months with sustained suppression. A 100 to 200 option can be considered after 3 to 6 months of suppression. Restart below 100, or at 100 to 200 with detectable HIV RNA.

0 of 1 answered
01Which patient needs primary TE prophylaxis?
Answer every question to submit.
196.05

Deliver Full CNS Induction

Preferred acute therapy pairs antiparasitic folate-pathway inhibition with protective rescue, or uses full-dose TMP-SMX. Treatment continues long enough to control brain tissue disease.

What to learn
  • Pyrimethamine
  • Sulfadiazine
  • Leucovorin
  • TMP-SMX
  • Six weeks
CNS evidence pathwayFull-dose induction to Treat at least 6 weeks
01Full-dose inductionDefine the starting evidence

Do not lose diagnostic context.

02Leucovorin rescueCarry the evidence forward

Do not lose diagnostic context.

03Measure responseCarry the evidence forward

Do not lose diagnostic context.

04Treat at least 6 weeksOwn the next decision

Document the clinical action.

Load pyrimethamine

Give 200 mg once. At 60 kg or less, continue 50 mg daily with sulfadiazine 1,000 mg every 6 hours. Above 60 kg, use pyrimethamine 75 mg daily with sulfadiazine 1,500 mg every 6 hours.

Rescue folate

Give leucovorin 10 to 25 mg daily with pyrimethamine and increase it when marrow toxicity requires. Leucovorin is part of the regimen, not an optional supplement.

Use TMP-SMX as a preferred option

Dose by the TMP component at 5 mg/kg with SMX 25 mg/kg IV or orally twice daily. Use it when pyrimethamine is delayed, unaffordable, inaccessible, or a poorer fit.

Treat the tissue course

Continue acute therapy for at least 6 weeks. Extend treatment when disease is extensive or clinical or radiographic response remains incomplete.

Transition without a gap

Every patient moves directly from induction to chronic maintenance until immune reconstitution is durable.

0 of 1 answered
01What is the correct pyrimethamine regimen for a 68 kg adult?
Answer every question to submit.
196.06

Preserve Coverage When the First Regimen Fails

An alternative must still treat TE, protect against PCP when needed, reach adequate exposure, and remain safe for marrow, kidney, liver, skin, and blood.

What to learn
  • Clindamycin
  • Atovaquone
  • G6PD
  • Sulfa allergy
  • Marrow toxicity
CNS evidence pathwayDefine intolerance to Monitor exposure
01Define intoleranceDefine the starting evidence

Do not lose diagnostic context.

02Preserve TE activityCarry the evidence forward

Do not lose diagnostic context.

03Preserve PCP coverageCarry the evidence forward

Do not lose diagnostic context.

04Monitor exposureOwn the next decision

Document the clinical action.

Use the preferred sulfa alternative

Pyrimethamine plus leucovorin plus clindamycin 600 mg every 6 hours treats acute TE when sulfadiazine cannot be used. It does not prevent PCP, so add separate PCP prophylaxis.

Make atovaquone absorb

Atovaquone 1,500 mg twice daily can be used alone or with pyrimethamine and leucovorin or sulfadiazine. Give every dose with a meal or nutritional supplement because absorption varies widely.

Evaluate sulfa allergy

Rapid desensitization can be considered for selected patients. Use atovaquone during the process until therapeutic TMP-SMX dosing is reached.

Protect marrow

Pyrimethamine can cause anemia, neutropenia, and thrombocytopenia. Give leucovorin and monitor CBC closely, commonly at least twice weekly during induction.

Test G6PD before dapsone

Use another agent in G6PD deficiency. Monitor for hemolysis and methemoglobinemia, including symptoms that a routine pulse oximeter may not explain.

0 of 1 answered
01What must be added to pyrimethamine, leucovorin, and clindamycin maintenance?
Answer every question to submit.
196.07

Measure Response Before Declaring Failure

Most patients improve within 14 days. Failure is a diagnostic event, not permission to continue unmeasured empiricism.

What to learn
  • Neurologic examination
  • Ten to 14 days
  • Repeat imaging
  • Biopsy
  • Adjuncts
CNS evidence pathwayBaseline examination to Biopsy if nonresponse
01Baseline examinationDefine the starting evidence

Do not lose diagnostic context.

02Days 10 to 14Carry the evidence forward

Do not lose diagnostic context.

03Repeat imagingCarry the evidence forward

Do not lose diagnostic context.

04Biopsy if nonresponseOwn the next decision

Document the clinical action.

Expect a trajectory

About 90 percent of patients improve clinically within 14 days. Follow focal deficits, cognition, fever, seizure, function, lesion size, enhancement, and edema.

Trigger escalation

Clinical or radiologic deterioration in the first week despite adequate exposure, or no clinical improvement by 10 to 14 days, should prompt diagnostic reassessment and strong consideration of brain biopsy.

Use steroids narrowly

Give dexamethasone only for clinically important edema or mass effect and stop as soon as feasible. Steroids can worsen immunosuppression and temporarily improve CNS lymphoma.

Treat seizures, not theoretical seizures

Give antiseizure medication after a TE-associated seizure and continue through acute therapy. Do not use routine prophylaxis without seizure.

Avoid interaction injury

Phenytoin, phenobarbital, carbamazepine, dexamethasone, and selected ARVs interact. Prefer a compatible seizure strategy and verify exposure.

0 of 1 answered
01When should brain biopsy be strongly considered?
Answer every question to submit.
196.08

Restore Immunity Without Losing CNS Discipline

ART controls the cause of susceptibility, but timing, interaction management, and inflammatory worsening require a coordinated plan.

What to learn
  • ART timing
  • Two to three weeks
  • IRIS
  • Interactions
  • Viral suppression
CNS evidence pathwayEstablish TE therapy to Keep diagnostic discipline
01Establish TE therapyDefine the starting evidence

Do not lose diagnostic context.

02Start ART in 2 to 3 weeksCarry the evidence forward

Do not lose diagnostic context.

03Watch inflammationCarry the evidence forward

Do not lose diagnostic context.

04Keep diagnostic disciplineOwn the next decision

Document the clinical action.

Use the practical timing

No randomized trial defines an exact date, but ART is generally started within 2 to 3 weeks after TE diagnosis once acute therapy is established.

Map interactions

Review anticonvulsants, dexamethasone, marrow suppressants, renal drugs, and the entire ART regimen. Enzyme inducers can lower ARV exposure and threaten suppression.

Recognize uncommon IRIS

TE IRIS can cause enlarging enhancing lesions, edema, or neurologic worsening after ART. It is uncommon, so verify drug exposure and reconsider the diagnosis before labeling it.

Maintain core therapy

Continue effective ART and anti-Toxoplasma treatment through IRIS when feasible. Use corticosteroids only for clinically significant inflammation and taper promptly.

0 of 1 answered
01When is ART generally initiated after TE diagnosis?
Answer every question to submit.
196.09

Suppress Recurrence Until Immunity Returns

Acute therapy controls active lesions but does not remove latent cysts. Maintenance bridges the patient to durable immune reconstitution.

What to learn
  • Secondary prophylaxis
  • TMP-SMX
  • CD4 above 200
  • Six months
  • Restart below 200
CNS evidence pathwayComplete induction to Restart below 200
01Complete inductionDefine the starting evidence

Do not lose diagnostic context.

02Suppress latent infectionCarry the evidence forward

Do not lose diagnostic context.

03CD4 above 200 for 6 monthsCarry the evidence forward

Do not lose diagnostic context.

04Restart below 200Own the next decision

Document the clinical action.

Begin immediately

Start chronic maintenance after acute treatment. Preferred options are pyrimethamine 25 to 50 mg daily plus sulfadiazine 2,000 to 4,000 mg daily and leucovorin 10 to 25 mg daily, or TMP-SMX DS twice daily.

Preserve PCP coverage

Pyrimethamine, sulfadiazine, and leucovorin and TMP-SMX both protect against PCP. A clindamycin maintenance regimen requires a separate PCP agent.

Stop only after durable recovery

Complete induction, remain asymptomatic, and sustain CD4 above 200 cells/mm3 for more than 6 months in response to ART before stopping.

Restart at the secondary threshold

Restart maintenance if CD4 falls below 200 regardless of HIV RNA. This is stricter than the below-100 threshold for primary prophylaxis because prior TE predicts recurrence.

Use imaging as context

Some specialists obtain MRI before stopping. Residual enhancement can persist despite successful treatment, so imaging supplements rather than replaces clinical and immune evidence.

0 of 1 answered
01When should chronic TE maintenance be restarted?
Answer every question to submit.
196.10

Build Closed-Loop Neuroinfectious Care

TE succeeds when diagnostic evidence, medication access, response checkpoints, ART, toxicity, and relapse prevention remain connected across care settings.

What to learn
  • Result ownership
  • Access
  • Neurologic monitoring
  • ART
  • Maintenance
CNS evidence pathwayRecognize to Prevent relapse
01RecognizeDefine the starting evidence

Do not lose diagnostic context.

02TreatCarry the evidence forward

Do not lose diagnostic context.

03Restore immunityCarry the evidence forward

Do not lose diagnostic context.

04Prevent relapseOwn the next decision

Document the clinical action.

Own the first 24 hours

Stabilize airway, seizure, and mass effect, obtain contrast imaging, collect safe diagnostic studies, start full-dose therapy, and document the baseline neurologic examination.

Own access and exposure

Secure pyrimethamine or use TMP-SMX without delay, calculate weight-based doses, give leucovorin, pair atovaquone with food, and reconcile every interacting drug.

Own the response date

Schedule neurologic reassessment and repeat imaging, with a defined 10-to-14-day threshold for biopsy or diagnostic escalation.

Own immunity

Start ART in the planned window, confirm viral suppression and CD4 recovery, and distinguish IRIS from failure using evidence.

Own relapse prevention

Deliver maintenance before discharge, monitor toxicity and adherence, and document stopping and restart criteria.

0 of 1 answered
01Which plan best represents closed-loop TE care?
Answer every question to submit.

Check the connections.

Each attempt draws 10 questions from the complete 136 question bank.

136 questions in this module bank10 questions per attempt

Each attempt draws a fresh set and rearranges the answer choices.

Current clinical foundation.

Lecture material was synthesized with the following contemporary guidance. Verify local policy and current guidance before applying clinical information.

  1. NIH Toxoplasma gondii Encephalitis Adult and Adolescent OI Guideline
  2. NIH OI Primary Prophylaxis Table
  3. NIH OI Treatment Table
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